Posts Tagged ‘external gills’

Hetaerina americana exuvia

March 18, 2018

An American Rubyspot damselfly (Hetaerina americananymph was collected by Bob Perkins on 06 August 2017 along the New River in Grayson County, Virginia USA. The nymph was reared in captivity, albeit briefly, until it emerged on 09 August 2017.

Pattern recognition can be used to tentatively identify damselfly larvae/exuviae to the family level: the shape of the prementum is characteristic for each of the three families of damselflies that occur in the mid-Atlantic region of the United States of America; mnemonics can be used to remember each distinctive shape.

Family Calopterygidae (Broad-winged Damselflies) features a prementum with a shape that looks somewhat similar to Family Coenagrionidae (Narrow-winged Damselflies). Look for an embedded raindrop shape, located toward the upper-center of the prementum.

Also notice another field mark for Calopterygidae: the first antenna segment is equal to or longer than the length of the other six (6) segments added together. (Editor’s Note: Some of the smaller antennae segments are missing. It’s likely those delicate parts broke off during shipping and/or cleaning.)

No. 2 | Hetaerina americana | exuvia (ventral)

Two field marks verify the genus and species of this specimen as Hetaerina americana: the labial cleft extends only to the base of the palpal lobes, as shown in Photo No. 1; and the external gills are 8.5 mm to 10 mm long (Daigle, 1991), as shown in Photo No. 2.

Before and after

Photo No. 3 shows a dorsal view of the exuvia before it was cleaned in order to remove unknown fibers covering the body and dirt/debris that obscured the labial cleft in the prementum.

No. 3 | Hetaerina americana | exuvia (dorsal)

Photo No. 1, 2 and 4 show the exuvia after cleaning. The operation appears to have been successful, other than collateral damage to two legs.

No. 4 | Hetaerina americana | exuvia (dorsal)

The next photograph shows the damselfly during emergence from one of Bob Perkins‘ holding tanks. Good timing, Bob!

Image used with permission from Bob Perkins.

The last photo shows the adult American Rubyspot damselfly sometime after emergence. Hetaerina americana is 38-46 mm in total length (Paulson, 2011). This individual is a male, as indicated by its hamules and terminal appendages.

Image used with permission from Bob Perkins.

Related Resource: Florida Damselflies (Zygoptera) – A Species Key to the Aquatic Larval Stages, by Jerrell James Daigle. Technical Series, Volume 11, Number 1, December 1991. State of Florida, Department of Environmental Regulation.

Tech Tips

The following equipment was used to shoot Photo No. 2, 3 and 4: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); Canon MT-26EX-RT Macro Twin Lite. Photo No. 1 , 2 and 4: the Canon MT-26 was set for “Master” mode, and Canon 580 EX- and Canon 580EX II Speedlites were set for “Slave” mode. A Canon MP-E 65mm Macro lens (manual focus only, set for 2x magnification) plus multiple-flash setup was used for Photo No. 1.

Adobe Photoshop CC 2017 was used to annotate selected images.

Bob Perkins’ photos were shot using a Canon EOS Rebel T3i camera body and Canon EF-S 60mm macro lens.

Copyright © 2018 Walter Sanford. All rights reserved.

American Rubyspot damselfly exuvia

March 12, 2018

An American Rubyspot damselfly (Hetaerina americana) nymph was collected by Bob Perkins on 06 August 2017 along the New River in Grayson County, Virginia USA. The nymph was reared in captivity, albeit briefly, until it emerged on 09 August 2017.

This individual is a member of the Family Calopterygidae (Broad-winged Damselflies), as indicated by the length of antenna segment 1 (shown below). See Hetaerina americana exuvia for a more detailed explanation.

No. 1 | Hetaerina americana | exuvia (dorsal)

If you look closely at the full-size version of both photos, then you should notice the specimen is covered by a lot of fibers of unknown origin.

That nymph was in the final instar when I collected it. Therefore it didn’t shed the algae and debris that had collected on its bristles. Source Credit: Personal communication with Bob Perkins.

A quick look at the prementum (below) shows the labial cleft, a key field mark for verifying the species, is obscured by some of the “debris” that Bob mentioned. As of this writing, the exuvia is soaking in a soapy water bath in the hope that it can be cleaned sufficiently to see that field mark clearly.

No. 2 | Hetaerina americana | exuvia (ventral)

To be continued

Tech Tips

The following equipment was used to shoot Photo No. 1 and 2: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite.

Two Sunpak LED-160 Video Lights (each with a white translucent plastic filter) were used for both photos.

Adobe Photoshop CC 2017 was used to annotate both images.

Copyright © 2018 Walter Sanford. All rights reserved.


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