Posts Tagged ‘nymph’

Cordulegaster sp. larva (dorsal view)

February 25, 2019

This post features a focus-stacked composite image that shows a dorsal view of an odonate larva/nymph from the Family Cordulegastridae (Spiketails) that was collected and reared by Bob Perkins. The larva died before it metamorphosed into an adult.

Cordulegaster sp. larva (female) | dorsal view

Most larvae go through 10-13 stages of development known as “instars.” The author lacks sufficient experience to identify the instar of this specimen, although it appears to be one of the later stages as indicated by its well-developed wing pads.

Related Resources

Tech Tips

12 photos were used to create the focus stack. A single focus point was positioned over select anatomical features; photos were taken at each point of interest.

The following equipment was used to shoot all of the photographs for the focus-stacked composite image, shown above: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to create the focus-stacked composite image, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

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Cordulegaster sp. larva (ventral view)

February 22, 2019

Bob Perkins collected and reared an odonate larva/nymph from the Family Cordulegastridae (Spiketails). The larva died before it metamorphosed into an adult.

This post features a focus-stacked composite image that shows a ventral view of the preserved larva; a composite image showing the dorsal view will be published in my next blog post.

Cordulegaster sp. larva (female) | ventral view

This individual is a female, as indicated by her rudimentary ovipositor that can be seen on the ventral side of the specimen along the boundary between abdominal segments eight and nine (S8-9). Do you see it?

Related Resources

Tech Tips

Nine (9) photos were used to create the focus stack. A single focus point was positioned over select anatomical features; photos were taken at each point of interest.

The following equipment was used to shoot all of the photographs for the focus-stacked composite image, shown above: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to create the focus-stacked composite image, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

“Generic Baskettail” (definitely not a Cruiser)

February 18, 2019

larva/nymph in the Family Corduliidae (Emeralds) was collected by Bob Perkins on 02 December 2017 from a pond in Orange Park, Florida (USA). The larva died before it metamorphosed into an adult.

As you can see by looking at a close-up image of the face-head at 3x magnification, there is no horn on the face of the specimen. Therefore this individual is not a member of Family Macromiidae (Cruisers), as I speculated in my last blog post.

“Generic Baskettail” larva (preserved specimen) | face-head

Knowing the limits of our expertise

Although I still need to key out the specimen carefully, at this point I’m certain Bob is correct — the larva is a member of the Family Corduliidae (Emeralds). The question that remains unanswered is “Which genus/species?” We may never know the answer, as Bob and I have reached the limit of our experience and expertise.

I did a quick scan of Paulson’s [book], looking at the Emerald Family. Here, according to the range maps, are the possibilities for Orange Park [FL]. I believe you can see why I stopped at “generic basketttail.” Source Credit: Bob Perkins.

What do you think the identity is? Most of the items in the preceding species list feature links to photos of odonate larvae/exuviae. See the links to BugGuide from the scientific names in the list.

Related Resource: Test shots: “Generic Baskettail?”

Tech Tips

Four (4) photos were used to create the preceding focus-stacked composite image. A single focus point was positioned over the face, between the antennae. At a magnification ratio of 3:1, it’s difficult to manually focus on a single point — the slightest movement around the macro rig changes focus unintentionally. A simple work-around for this problem is to take several shots of the same focus point and create a composite image of the photos.

The following equipment was used to shoot the preceding composite image: Canon EOS 5D Mark II digital camera, in manual mode; Canon MP-E 65mm Macro lens (set for f/16 at 3x); a Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and a single external flash set for “Slave” mode — a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier. A Sunpak LED-160 Video Light was used to add fill light to the top of the subject.

Auto power-off was disabled for the camera and external flash units.

Adobe Photoshop CC 2017 was used to create the focus stack, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

Test shots: “Generic Baskettail?”

February 15, 2019

larva/nymph in the Family Corduliidae (Emeralds) was collected by Bob Perkins on 02 December 2017 from a pond in Orange Park, Florida (USA). The larva died before it metamorphosed into an adult.

Test shots of this beautifully preserved specimen were taken using a small-ish aperture of f/11 for greater depth of field. The following photos are “one-offs,” that is, not composite images.

Dorsal

A single focus point — located on the thorax (specifcally, the “shoulder pad” along the right side of the body) — was used to shoot this photo. The specimen has enough “relief” that focus on the wing pads and dorsal hooks is slightly soft. This view of the larva is a good candidate for focus-stacking.

The terminal appendages (cerci, epiproct, paraprocts) are shown clearly in the following photo.

“Generic Baskettail” larva (preserved specimen) | Orange Park, FL USA

Bob’s best guess of the identity of the specimen is Epitheca sp., either Common Baskettail (Epitheca cynosura) or Prince Baskettail (Epitheca princeps).

Whenever I see an odonate larvae/exuviae with long legs, my first thought is Family Macromiidae (Cruisers). Then I check for a horn on top of the head, a key field marker for Cruisers. Look closely at the dorsal view of the larva and I think you’ll agree with me there appears to be a horn on the head. I would like to take close-up photos of the head and key out the specimen in order to determine its identity. In the meantime, my best guess is Stream Cruiser (Didmops transversa) as indicated by the lateral spines on abdominal segment nine (S9) and the absence of a dorsal hook on S10.

Ventral

The ventral side of the specimen has almost no “relief,” so a “one-off” focused on the thorax looks fairly good from head-to-tail.

“Generic Baskettail” larva (preserved specimen) | Orange Park, FL USA

Related Resource: “Generic Baskettail” (definitely not a Cruiser)

Tech Tips

The following equipment (shown below) was used to shoot the preceding photos: Fujifilm X-T1 digital camera; Fujifilm MCEX-16 extension tube; Fujinon XF80mm macro lensGodox XProF TTL Wireless Flash Trigger for Fujifilm camerasGodox TT685F Thinklite TTL Flash for Fujifilm CamerasGodox TT685C Thinklite TTL Flash for Canon Cameras fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier; and a Canon 580EX II Speedlite mounted on a Godox X1R-C TTL Wireless Flash Trigger Receiver for Canon. A new Godox TT685O Thinklite TTL Flash for Olympus/Panasonic Cameras was added to an array of radio-controlled external flash units used to light the specimen. All flashes were set for Manual Mode at 1/128 power.

Adobe Photoshop CC 2017 was used to spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

Test shot: Cordulegaster sp. larva

February 1, 2019

Bob Perkins collected and reared a larva/nymph from the Family Cordulegastridae (Spiketails). The larva died before it metamorphosed into an adult.

Test shots of this beautifully preserved specimen (Cordulegaster sp.) were taken using a relatively small aperture of f/16 for greater depth of field. The following photo is a “one-off,” that is, not a composite image.

Cordulegaster sp. larva (preserved specimen) | face-head

Odonates are aquatic insects. They spend most of their life as larvae that live in water; this stage of their life cycle can last from a few months to a few years. Finally, they emerge from the water and metamorphose into adults in order to reproduce; their offspring return to the water and the cycle begins again.

Most larvae go through 10-13 stages of development known as “instars.” The author lacks sufficient experience to identify the instar of this specimen, although it appears to be one of the later stages.

Related Resources

Tech Tips

The following equipment was used to shoot the preceding photo: Canon EOS 5D Mark II digital camera, in manual mode; Canon MP-E 65mm Macro lens (set for 3x); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier. A Sunpak LED-160 Video Light was used to light the underside of the white plastic posing “stage.”

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

More test shots: Stylogomphus albistylus exuvia

December 21, 2018

This blog post features another focus-stacked composite image of an Eastern Least Clubtail dragonfly (Stylogomphus albistylus) exuvia from an odonate nymph that Bob Perkins collected and reared.

11 photos were used to create this focus stack. My camera is set for spot focus, meaning one-area focus for each photo. A single focus point was positioned manually somewhere on the face-head in all 11 photos.

From this viewpoint, the large, dish-shaped antennae remind me of wooden spoons (with short handles).

This is the first time I shot some photos in portrait mode using my new 3 Legged Thing QR11-LC Universal L-Bracket (Orange) mounted on my Canon 5D Mark II DSLR camera. I’m sold!

3 Legged Thing QR11-LC Universal L-Bracket (Orange).

Related Resources

Tech Tips

The following equipment was used to shoot all of the photographs for the focus-stacked composite image of the exuvia: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Adobe Photoshop CC 2017 was used to create the focus-stacked composite image, as well as spot-heal and sharpen the final output.

I used my Panasonic Lumix DMC-FZ150 superzoom camera and a handheld Canon 580EX Speedlite fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier to shoot the photo of my Canon 5D Mark II.

Copyright © 2018 Walter Sanford. All rights reserved.

Test shots: Stylogomphus albistylus exuvia

December 19, 2018

Bob Perkins collected and reared an Eastern Least Clubtail dragonfly (Stylogomphus albistylus) nymph. This blog post features two focus-stacked composite images of the exuvia from the odonate nymph.

Nine photos were used to create the first focus stack. This small specimen features several easy-to-recognize field marks including large, dish-shaped antennae, distinctive color bands on the legs, lateral spines on abdominal segments eight and nine (S8-9), and terminal appendages tipped with a lighter color.

12 photos were used to create the last focus stack. This individual might be a male, as indicated by what appear to be vestigial hamules on the ventral side of the specimen.

Tech Tips

The following equipment was used to shoot all of the photographs for the two focus-stacked composite images, shown above: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Adobe Photoshop CC 2017 was used to create the focus-stacked composite images, as well as spot-heal and sharpen the final output.

Copyright © 2018 Walter Sanford. All rights reserved.

Swift River Cruiser exuvia

April 25, 2018

A late-stage emergent teneral female Swift River Cruiser dragonfly (Macromia illinoiensis) was spotted on 27 May 2017 along the Potomac River at Riverbend Park in Fairfax County, Virginia USA. The exuvia was collected, with permission from park staff, after the female flew away from the place where she metamorphosed from a nymph to an adult.

No. 1 | 27 MAY 2017 | Riverbend Park | Swift River Cruiser (female)

The next image is a composite of 35 photos. The specimen is perfectly in focus from head-to-tail, including the legs.

The last image is a composite of eight photos. The focus point for each photo in the set is limited to the body only. Surprisingly, all six legs are acceptably in focus except for the tip of the left hind leg.

The official early-date for Swift River Cruiser dragonfly is 08 May in the Commonwealth of Virginia. Since the early-date for Royal River Cruiser dragonfly (Macromia taeniolata) is 15 May, the exuvia helps to confirm the identity of the adult is Swift River Cruiser. 10 October is the late-date for both species.

Tech Tips

Photo No. 1 was taken using my Panasonic Lumix DMC-FZ150 superzoom bridge camera plus Canon 580EX Speedlite, my go-to kit for photowalking.

The following equipment was used to shoot Photo No. 2 and 3: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and Canon 580 EX- and Canon 580EX II Speedlites set for “Slave” mode.

Photo No. 2-3 are focus-stacked composite images created using Adobe Photoshop CC 2017.

Related Resource: Swift River Cruiser (emergent female).

Copyright © 2018 Walter Sanford. All rights reserved.

Another focus stacking face-off

April 19, 2018

Here’s another face-off between a single macro photo and a focus-stacked composite image. Let’s start with the composite image this time.

The first example is a composite image created from 14 photos.

In a recent blog post, I wrote…

My goal is to shoot the fewest number of photos (using a relatively small aperture such as f/18) that will show the entire specimen in focus when the photo set is focus-stacked to create a composite image. Source Credit: More Calico Pennant exuvia composite images.

I used to shoot several photos of a single focus point, e.g., the prementum, and select the sharpest image for editing/focus stacking. Now I’m using a wider aperture such as either f/11 or f/8 (for sharpness), shooting more photos, and using every photo that I take. My rationale is simple: A single photo may not be the sharpest photo of a single focus point, but it probably shows other areas that are in focus. In this case, I think more “raw material” is better than less.

The last example is one of the better photos from the set of 14. When you click on the images they open in a new tab automatically. Toggle back-and-forth between tabs and I think you will agree the composite image is clearly better than the following single photo.

The Backstory

An American Rubyspot damselfly (Hetaerina americananymph was collected by Bob Perkins on 06 August 2017 along the New River in Grayson County, Virginia USA. The nymph was reared in captivity, albeit briefly, until it emerged on 09 August 2017.

Copyright © 2018 Walter Sanford. All rights reserved.

Why focus stack macro photos?

April 17, 2018

Why focus stack macro photos? The answer is obvious: The difference between a single macro photo and a focus-stacked composite image is like night and day.

The first example is one of the better photos from a set of 13. It is the same photo that is featured in Hetaerina americana exuvia, my identification guide for American Rubyspot damselfly exuviae.

The last example is a composite image created using all 13 photos in the set.

You may not notice the difference in quality unless you look at the full-size version of both images. When you click on the images they open in a new tab automatically. Toggle back-and-forth between tabs and I think you will agree the composite image is clearly better than the single photo.

The Backstory

An American Rubyspot damselfly (Hetaerina americananymph was collected by Bob Perkins on 06 August 2017 along the New River in Grayson County, Virginia USA. The nymph was reared in captivity, albeit briefly, until it emerged on 09 August 2017.

Copyright © 2018 Walter Sanford. All rights reserved.


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