Archive for the ‘Fujinon XF80mm macro lens’ Category

Macromia illinoiensis exuvia (face-head)

May 20, 2020

The following photograph of a Swift River Cruiser dragonfly (Macromia illinoiensis) exuvia shows a prominent horn on the face that is a key field mark for larvae/exuviae in the Family Macromiidae (Cruisers).

The specimen was collected, with permission from park staff, on 27 May 2017 along the Potomac River at Riverbend Park in Fairfax County, Virginia USA.

Tech Tips

The subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique. The specimen was posed on its dorsal side, so the camera was focused on the face-head-ventral view of the exuvia. The final image was cropped and rotated 180° during post-processing.

10 photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. RAW FILE CONVERTER EX 3.0 was used to convert Fujifilm RAF files to TIFF files. Adobe Photoshop CC 2017 was used to create a focus-stacked composite image that was edited using Apple Aperture.

Editor’s Note: This blog post is the last installment in what turned out to be a three-part series. The featured focus-stacked composite image is a little closer to what I had in mind when I set up the photo shoot.

  1. MYN – Macromiidae larvae/exuviae are horny
  2. RAW FILE CONVERTER EX 3.0

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Macromiidae larvae/exuviae are horny

May 15, 2020

A prominent horn on the face is a key field mark for larvae/exuviae in the Family Macromiidae (Cruisers), as shown in the following photograph of a Swift River Cruiser dragonfly (Macromia illinoiensis) exuvia.

The base of the triangular horn is located above the labium (face mask), between the long, thin antennae.

The specimen was collected, with permission from park staff, on 27 May 2017 along the Potomac River at Riverbend Park in Fairfax County, Virginia USA.

Tech Tips

The subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique. The specimen was posed on its dorsal side, so the camera was focused on the face-head-ventral view of the exuvia. The image was rotated 180° during post-processing.

My vintage 2009 Apple iMac desktop computer is too old to support drivers for importing RAF files from my Fujifilm X-T3 mirrorless digital camera into either Apple Aperture or Adobe Lightroom.

The work-around is to use a free application from Fujifilm that converts RAF files to TIFF files, which can be opened and edited with Aperture and Lightroom. Problem is I’m a big procrastinator and haven’t learned how to use the file converter application, so I simply edited one of the JPGs straight from the camera.

For the most part, the finished image looks fairly good although the eyes are blown out a little to a lot. JPG files have less dynamic range than RAF files so I was unable to recover the blown highlights.

Hmmm, it might be time to buy a new desktop computer!

Post Updates

I finally got around to figuring out how to use the free Fujifilm application to convert RAF files to TIFFs. See my follow-up blog post entitled “RAW FILE CONVERTER EX 3.0” for step-by-step instructions.

10 photos (converted from RAF to TIFF), including the one-off featured in this blog post, were used to create a focus-stacked composite image of the specimen that is a little closer to what I had in mind when I set up the photo shoot.

Copyright © 2020 Walter Sanford. All rights reserved.

Focus bracketing using Fujifilm X-T3

May 11, 2020

In-camera focus bracketing was used to shoot 35 photos automatically. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown below.

Focus-stacked composite image

The toy is a Poliwrath, one of many Pokemon characters. The 7″ plastic ruler is from the Calvert Marine Museum. Do you know why the small ruler is 7″ long rather than the more common 6″ length? Please leave a comment if you know the correct answer.

Photo No. 1

The camera was focused manually on the closest point in the foreground, before pressing the shutter button.

80mm (120mm, 35mm equivalent) | f/8 | 1/250s | ISO 160 | 0 ev

Select photos from the 35-photo set show the focus point advancing automatically from the foreground to background.

Photo No. 5

80mm (120mm, 35mm equivalent) | f/8 | 1/250s | ISO 160 | 0 ev

Photo No. 20

80mm (120mm, 35mm equivalent) | f/8 | 1/250s | ISO 160 | 0 ev

Photo No. 35

80mm (120mm, 35mm equivalent) | f/8 | 1/250s | ISO 160 | 0 ev

Related Resources

Tech Tips

Minimal effort was invested in arranging the subjects and lighting the scene.

Some trial and error is required in order to determine the correct focus bracket settings for a given combination of camera and lens, in this case, my Fujifilm X-T3 and 80mm macro lens.

As it turns out, I shot six sets of test shots before I found settings that worked the way I wanted. The following settings were used to shoot the 35 photos for the focus-stacked composite image featured in this post: Frames = 35; Step = 10; Interval = 3 s.

The camera was set to save files as FINE+RAW. In the interest of expediency, the composite image was created using JPGs straight from the camera. Photo No. 1, 5, 20, and 35 are also unedited JPGs.

What are the take-aways?

The technique works as advertised and should help to save time by automating the most tedious part of the process of creating focus stacks. Further experimentation is planned.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Hylogomphus adelphus exuvia (dorsal)

May 4, 2020

The following Anisoptera exuvia — collected by Freda van den Broek on 10 June 2019 along the St. Croix River in Polk County, Wisconsin USA — is definitely a member of Family Gomphidae (Clubtails), probably Mustached Clubtail (Hylogomphus adelphus).

Dorsal view

The abdomen is wider than the head. Vestigial mid-dorsal hooks are noticeable along several abdominal segments, especially segment nine (S9). A “median groove” is apparent along part of the abdomen. The lateral spine on S9 is spinulose-serrate along the outer edge.

10 JUN 2019 | Polk County, WI | H. adelphus exuvia (dorsal)

Related Resources

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

15 photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create- and annotate the focus-stacked composite image shown above.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Hylogomphus adelphus exuvia (ventral)

May 1, 2020

The following Anisoptera exuvia — collected by Freda van den Broek on 10 June 2019 along the St. Croix River in Polk County, Wisconsin USA — is definitely a member of Family Gomphidae (Clubtails), probably Mustached Clubtail (Hylogomphus adelphus).

Ventral view

Notice the exuvia has a flat labium (prementum). There are lateral spines located on abdominal segments six through nine (S6-S9); the lateral spine on S9 is spinulose-serrate along the outer edge. Also notice the well-developed tibial burrowing hooks on its legs.

10 JUN 2019 | Polk County, WI | H. adelphus exuvia (ventral)

Male (vestigial genitalia)

Male odonates have two sets of sex organs: primary genitalia located on abdominal segment nine (S9); and secondary genitalia located on abdominal segments two-to-three (S2-3).

Both sets of vestigial genitalia are clearly visible on the ventral side of some (but not all) specimens, such as the H. adelphus exuvia shown above.

Related Resources

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

12 photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. A subset of six (6) photos and Adobe Photoshop CC 2017 were used to create- and annotate the focus-stacked composite image shown above.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Hylogomphus adelphus exuvia

April 29, 2020

An Anisoptera exuvia was collected by Freda van den Broek on 10 June 2019 along the St. Croix River in Polk County, Wisconsin USA.

This specimen is definitely a member of Family Gomphidae (Clubtails), probably Mustached Clubtail (Hylogomphus adelphus).

10 JUN 2019 | Polk County, WI | Anisoptera exuvia (face-head-dorsal)

Related Resources

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique. This photo is a “one-off,” that is, not a composite image.

Eight (8) photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 will be used to create a focus-stacked composite image of the exuvia.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Too dark or too light?

April 27, 2020

The following Anisoptera exuvia — collected by Freda van den Broek on 10 June 2019 along the Red Cedar River in Barron County, Wisconsin USA — is definitely a member of Family Gomphidae (Clubtails), possibly Rusty Snaketail (Ophiogomphus rupinsulensis).

Dorsal-lateral view

Nine (9) photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown below.

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (dorsallateral)

When I previewed the RAW files used to create the preceding composite image, I thought most of the files look too dark.

So I did a do-over using 15 photos, resulting in a new version of the composite that is perfectly in focus but a little too light. Compare/contrast the two composite images and you should notice it’s easier to see the mid-dorsal hooks along the abdomen in the first image. The primary purpose for the images is to illustrate those field marks, therefore I think the first (darker) image is better.

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (dorsallateral)

Face-head-dorsal view

Five (5) photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown below.

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (face-head-dorsal)

My first impression of the version shown above is it’s too dark, and I second-guessed myself for not shooting more photos for the focus stack.

I created a new focus-stacked composite image using a little more light and 14 photos. When I examined the RAW files used to create the new composite image (shown below), I thought most of the files still look too dark. (But I was wrong.)

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (face-head-dorsal)

So I created a third version using much more light and 24 photos. Although it’s debatable which image looks best, the first- and second images show a few key field marks of the antennae better than the third image.

It turns out the antennae are one indicator this specimen is from the genus Ophiogomphus (Snaketails), so the first two (darker) images are the winners once again.

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (face-head-dorsal)

For what it’s worth, the second of three images is my favorite as indicated by the fact that the image is cropped so there’s room for annotations.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Clubtail exuvia (dorsal-lateral view)

April 24, 2020

The following Anisoptera exuvia — collected by Freda van den Broek on 10 June 2019 along the Red Cedar River in Barron County, Wisconsin USA — is definitely a member of Family Gomphidae (Clubtails), possibly Rusty Snaketail (Ophiogomphus rupinsulensis).

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (dorsallateral)

Notice the exuvia has little stubby mid-dorsal hooks along the length of its abdomen, and lateral spines on abdominal segments seven through nine (S7-S9).

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

15 photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown above.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Clubtail exuvia (ventral view)

April 22, 2020

An Anisoptera exuvia was collected by Freda van den Broek on 10 June 2019 along the Red Cedar River in Barron County, Wisconsin USA.

This specimen is definitely a member of Family Gomphidae (Clubtails), possibly Rusty Snaketail (Ophiogomphus rupinsulensis).

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (ventral)

Notice the exuvia has a flat labium (prementum). There are lateral spines located on abdominal segments seven through nine (S7-S9).

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

Seven (7) photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown above.

Copyright © 2020 Walter Sanford. All rights reserved.

Cobra Clubtail versus Midland Clubtail

April 20, 2020

Odonate exuviae from two species in Genus GomphurusCobra Clubtail (Gomphurus vastus) and Midland Clubtail (Gomphurus fraternus) — are nearly perfect body doubles, with the exception of one key field mark that can be used to differentiate the species.

Dorsal views

Lateral spines are located on abdominal segments six through nine (S6-S9) for both species.

10 JUN 2019 | Barron County, WI | Midland Clubtail exuvia (dorsal)

Ventral views

The overall shape of the prementum is similar for both species.

10 JUN 2019 | Barron County, WI | Midland Clubtail exuvia (ventral)

Prementum (ventral view)

The shape of the palpal lobes on the prementum is different for the two species, as shown in the following diagram on p. 15, Key to the species of genus GomphurusIdentification Keys to Northeastern Anisoptera Larvae, compiled by Ken Soltesz.

The following images are magnified approximately three and one-half times life size (~3.5x).

For both species, focus on the row of teeth along the right palpal lobe. Notice the strongly curved shape of the palpal lobe for Cobra Clubtail (shown above), in contrast with the gently arched shape of the palpal lobe for Midland Clubtail (shown below). Also notice the Cobra palpal lobe has fewer teeth than Midland.

10 JUN 2019 | Barron County, WI | Gomphurus fraternus (exuvia)

Adults

Adult Cobra Clubtail dragonflies are almost identical to Midland Clubtails too. Several field marks can be used to differentiate the two species. In my opinion, one field mark is the easiest to recognize where it really matters — in the field!

16 MAY 2016 | Riverbend Park | Cobra Clubtail (adult male)

Notice there aren’t any mid-dorsal marks on abdominal segments eight and nine (S8-S9) for Cobra Clubtail (shown above). In contrast, there is a small yellow triangle on abdominal segment eight (S8) for Midland Clubtail (shown below). This is true for males and females of both species.

Photo used with permission from Freda van den Broek.

Copyright © 2020 Walter Sanford. All rights reserved.


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