Archive for the ‘Canon MT-26EX-RT Macro Twin Lite’ Category

Composite image: “Generic Gomphid” (face)

February 27, 2019

larva/nymph in the Family Gomphidae (Clubtails) was collected by Bob Perkins from the New River in southwestern Virginia. The larva died before it metamorphosed into an adult.

“Generic Gomphid” larva (preserved specimen) | New River, VA USA

An Ashy Clubtail dragonfly nymph was also collected by Bob Perkins. (The date and location where the specimen was collected are unknown.) The nymph was reared in captivity until it emerged on 21 March 2017 and metamorphosed into an adult female. This specimen is the exuvia from the nymph.

Although face-head of the “Generic Gomphid” and Ashy Clubtail look similar, they aren’t identical. More later after the specimen is keyed out carefully.

Related Resources

Tech Tips

13 photos were used to create the focus stack of the “Generic Gomphid.” A single focus point was positioned over select anatomical features; photos were taken at each point of interest.

The following equipment was used to shoot the composite image of the “Generic Gomphid”: Canon EOS 5D Mark II digital camera, in manual mode; Canon MP-E 65mm Macro lens (set for f/11 at ~2.5x); a Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and a single external flash set for “Slave” mode — a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and external flash units.

Adobe Photoshop CC 2017 was used to create the focus stack, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

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Cordulegaster sp. larva (dorsal view)

February 25, 2019

This post features a focus-stacked composite image that shows a dorsal view of an odonate larva/nymph from the Family Cordulegastridae (Spiketails) that was collected and reared by Bob Perkins. The larva died before it metamorphosed into an adult.

Cordulegaster sp. larva (female) | dorsal view

Most larvae go through 10-13 stages of development known as “instars.” The author lacks sufficient experience to identify the instar of this specimen, although it appears to be one of the later stages as indicated by its well-developed wing pads.

Related Resources

Tech Tips

12 photos were used to create the focus stack. A single focus point was positioned over select anatomical features; photos were taken at each point of interest.

The following equipment was used to shoot all of the photographs for the focus-stacked composite image, shown above: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to create the focus-stacked composite image, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

Cordulegaster sp. larva (ventral view)

February 22, 2019

Bob Perkins collected and reared an odonate larva/nymph from the Family Cordulegastridae (Spiketails). The larva died before it metamorphosed into an adult.

This post features a focus-stacked composite image that shows a ventral view of the preserved larva; a composite image showing the dorsal view will be published in my next blog post.

Cordulegaster sp. larva (female) | ventral view

This individual is a female, as indicated by her rudimentary ovipositor that can be seen on the ventral side of the specimen along the boundary between abdominal segments eight and nine (S8-9). Do you see it?

Related Resources

Tech Tips

Nine (9) photos were used to create the focus stack. A single focus point was positioned over select anatomical features; photos were taken at each point of interest.

The following equipment was used to shoot all of the photographs for the focus-stacked composite image, shown above: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to create the focus-stacked composite image, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

“Generic Baskettail” (definitely not a Cruiser)

February 18, 2019

larva/nymph in the Family Corduliidae (Emeralds) was collected by Bob Perkins on 02 December 2017 from a pond in Orange Park, Florida (USA). The larva died before it metamorphosed into an adult.

As you can see by looking at a close-up image of the face-head at 3x magnification, there is no horn on the face of the specimen. Therefore this individual is not a member of Family Macromiidae (Cruisers), as I speculated in my last blog post.

“Generic Baskettail” larva (preserved specimen) | face-head

Knowing the limits of our expertise

Although I still need to key out the specimen carefully, at this point I’m certain Bob is correct — the larva is a member of the Family Corduliidae (Emeralds). The question that remains unanswered is “Which genus/species?” We may never know the answer, as Bob and I have reached the limit of our experience and expertise.

I did a quick scan of Paulson’s [book], looking at the Emerald Family. Here, according to the range maps, are the possibilities for Orange Park [FL]. I believe you can see why I stopped at “generic basketttail.” Source Credit: Bob Perkins.

What do you think the identity is? Most of the items in the preceding species list feature links to photos of odonate larvae/exuviae. See the links to BugGuide from the scientific names in the list.

Related Resource: Test shots: “Generic Baskettail?”

Tech Tips

Four (4) photos were used to create the preceding focus-stacked composite image. A single focus point was positioned over the face, between the antennae. At a magnification ratio of 3:1, it’s difficult to manually focus on a single point — the slightest movement around the macro rig changes focus unintentionally. A simple work-around for this problem is to take several shots of the same focus point and create a composite image of the photos.

The following equipment was used to shoot the preceding composite image: Canon EOS 5D Mark II digital camera, in manual mode; Canon MP-E 65mm Macro lens (set for f/16 at 3x); a Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and a single external flash set for “Slave” mode — a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier. A Sunpak LED-160 Video Light was used to add fill light to the top of the subject.

Auto power-off was disabled for the camera and external flash units.

Adobe Photoshop CC 2017 was used to create the focus stack, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

More composite images: P. obscurus exuvia

February 6, 2019

The following focus-stacked composite images show dorsal- and ventral views of the exuvia from a Common Sanddragon (Progomphus obscuruslarva that was collected and reared by Bob Perkins.

Here are some personal observations after examining the specimen carefully.

The front- and middle legs block the mentum (prementum). This specimen is a good candidate for rehydrating the exuvia and reposing its legs.

Related Resource: Composite image: Progomphus obscurus exuvia.

Tech Tips

Six (6) photos were used to create the first focus stack; seven (7) photos were used for the second. A single focus point was positioned over select anatomical features, working from back-to-front; photos were taken at each point of interest.

The following equipment was used to shoot all of the photographs for the two focus-stacked composite images, shown above: Canon EOS 5D Mark II digital camera, in manual mode; Kenko 20mm macro automatic extension tubeCanon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Adobe Photoshop CC 2017 was used to create the focus-stacked composite images, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

Composite image: Progomphus obscurus exuvia

February 4, 2019

The following focus-stacked composite image shows the exuvia from a Common Sanddragon (Progomphus obscurus) larva that was collected and reared by Bob Perkins.

I have 10s, maybe 100s, of Common Sanddragon exuviae in my collection, but have never seen one cleaner than this beautiful specimen. I didn’t realize P. obscurus larvae are so hairy!

Related Resource: More composite images: P. obscurus exuvia.

Tech Tips

11 photos were used to create the focus stack. A single focus point was positioned over select anatomical features, working from back-to-front; photos were taken at each point of interest.

The following equipment was used to shoot the preceding composite image: Canon EOS 5D Mark II digital camera, in manual mode; Canon MP-E 65mm Macro lens (set for f/11 at 3x); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to create the focus stack, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

Test shot: Cordulegaster sp. larva

February 1, 2019

Bob Perkins collected and reared a larva/nymph from the Family Cordulegastridae (Spiketails). The larva died before it metamorphosed into an adult.

Test shots of this beautifully preserved specimen (Cordulegaster sp.) were taken using a relatively small aperture of f/16 for greater depth of field. The following photo is a “one-off,” that is, not a composite image.

Cordulegaster sp. larva (preserved specimen) | face-head

Odonates are aquatic insects. They spend most of their life as larvae that live in water; this stage of their life cycle can last from a few months to a few years. Finally, they emerge from the water and metamorphose into adults in order to reproduce; their offspring return to the water and the cycle begins again.

Most larvae go through 10-13 stages of development known as “instars.” The author lacks sufficient experience to identify the instar of this specimen, although it appears to be one of the later stages.

Related Resources

Tech Tips

The following equipment was used to shoot the preceding photo: Canon EOS 5D Mark II digital camera, in manual mode; Canon MP-E 65mm Macro lens (set for 3x); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier. A Sunpak LED-160 Video Light was used to light the underside of the white plastic posing “stage.”

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

Composite image: Shadow Darner dragonfly

January 11, 2019

Bob Perkins collected and reared a Shadow Darner dragonfly (Aeshna umbrosa) larva/nymph. This blog post features a focus-stacked composite image of a beautifully preserved specimen of the adult that emerged from the larva.

This individual is a male, as indicated by his “indented” hind wings.

Shadow Darner (Aeshna umbrosa) | face-head-dorsal

Artifacts

In my experience, focus stacking either works or it doesn’t. When it doesn’t work, “artifacts” appear in the final output that can be caused by many factors. There are work-arounds that can be used to repair the artifacts with mixed success. (See Tech Tips/Related Resources.)

I shot 19 photographs that were used to create a focus-stacked composite image of the Shadow Darner dragonfly. There’s a lot going on in those photos that caused too many artifacts to publish the final output. Several of the more noticeable artifacts are shown below.

I created another composite image; I used as many photos as I could before the first artifacts appeared. As it turns out, only five of 19 photos were used in the do-over composite image (shown at the beginning of this blog post). Look closely — some of the same artifacts are also noticeable in the do-over version.

Related Resources

Tech Tips

The following equipment was used to shoot the preceding composite image: Canon EOS 5D Mark II digital camera, in manual mode; Kenko macro automatic extension tubes (12mm, 20mm, and 36mm)Canon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to spot-heal and sharpen the final output.

Tech Tips/Related Resources

Copyright © 2019 Walter Sanford. All rights reserved.

Test shots: Shadow Darner dragonfly

January 9, 2019

Bob Perkins collected and reared a Shadow Darner dragonfly (Aeshna umbrosa) larva/nymph. This is a preserved specimen of the adult that emerged from the larva.

Test shots of this beautiful specimen were taken using a relatively small aperture of f/16 for greater depth of field. The following photo is a “one-off,” that is, not a composite image.

Related Resource: Test shots: Aeshna umbrosa exuvia.

Tech Tips

The following equipment was used to shoot the preceding photograph: Canon EOS 5D Mark II digital camera, in manual mode; Kenko macro automatic extension tubes (12mm, 20mm, and 36mm)Canon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and all external flash units.

Adobe Photoshop CC 2017 was used to spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.

Focus-stacking with CamRanger

January 7, 2019

This blog post reports the result of more experimentation with completely automated focus stacking using CamRanger.

25 photos were used to create this focus-stacked composite image of an Eastern Least Clubtail dragonfly (Stylogomphus albistylus) exuvia from an odonate nymph that Bob Perkins collected and reared.

I own two Canon macro lenses: a Canon MP-E 65mm Macro lens; and a Canon EF100mm f/2.8L Macro lens.

The MP-E 65mm macro is manual focus only, and cannot be used with CamRanger to create automated focus-stacked composite images.

In contrast, the EF100mm macro can be used with CamRanger to create automated focus stacks. In order to increase the magnification of the EF100mm macro, I used the lens in combination with three Kenko macro automatic extension tubes: 12mm; 20mm; and 36mm.

Pros and Cons

My goal was to create a tight close-up showing the face-head only. On one hand I failed to achieve my goal due to less maximum magnification using the combination of EF100mm macro lens and extension tubes rather than the MP-E 65mm macro lens.

On the other hand I shot 25 photos in a little more than four minutes*, with the focus plane shifted in small increments from front to back along the subject — all without touching the camera after setting the initial focus point. That’s what I call success!

Related Resources

Tech Tips

The following equipment was used to shoot all of the photographs for the focus-stacked composite image of the exuvia: CamRanger hardware, and iOS app running on my iPad mini (with retina display); Canon EOS 5D Mark II digital camera, in manual mode; Kenko macro automatic extension tubes (12mm, 20mm, and 36mm)Canon EF100mm f/2.8L Macro lens (set for manual focus); and Canon MT-26EX-RT Macro Twin Lite set for “Master” mode, and several external flashes set for “Slave” mode including Canon 580 EX- and Canon 580EX II Speedlites and a Godox TT685C Thinklite TTL Flash fitted with a Lastolite Ezybox Speed-Lite 2 flash modifier.

Auto power-off was disabled for the camera and all external flash units. CamRanger was set for a *10-second delay between shots in order to allow sufficient time for the lens to change focus automatically, the camera rig to settle, and the flash units to power-cycle.

Adobe Photoshop CC 2017 was used to create the focus-stacked composite image, as well as spot-heal and sharpen the final output.

Copyright © 2019 Walter Sanford. All rights reserved.


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