MYN – Hits and misses

This blog post might have been called “New ways of doing the same old thing.” In other words, experimenting with new techniques for shooting sets of macro photos of a familiar  subject and new variations for creating focus-stacked composite images.

80mm (120mm, 35mm equivalent) | f/16 | 1/200s | ISO 160 | 0 ev

Tech Tips

In-camera focus bracketing was used to shoot a photo set with my Fujifilm X-T3 mirrorless digital camera and Fujinon 80mm macro lens (coupled with 11mm and 16mm extension tubes for a little additional magnification).

The camera lens was focused manually on the closest point on the face of the subject. The shutter button was pressed one time; the first photo was taken after a 10-second timer elapsed, then the focus point advanced automatically from the initial focus point to a far point on the subject in the background of the photo.

RAW FILE CONVERTER EX 3.0 was used to batch-convert the resulting 50 images from Fujifilm RAF files to TIFF files. Adobe Photoshop CC 2017 was used to create five sub-stacks (10 photos per sub-stack) that were combined into one focus-stacked composite image. The final image was edited using Apple Aperture.

Analyzing the results

Most of the “misses” were self-inflicted.

For example, minimal effort was invested in arranging the subject and lighting the scene. Generally speaking, better lighting results in better photos.

Some trial and error is required in order to determine the correct focus bracketing settings for a given combination of camera and lens. The following settings were used to shoot the photo set for the focus-stacked composite image featured in this post: Frames = 50; Step = 10; Interval = 4 s.

Step size is a number from one (1) to 10, with one being the smallest increment and 10 being the largest. Although a step size of 10 enabled the camera to cover the subject completely from front-to-back in 50 frames, selecting the coarsest step increment might have resulted in small “focus gaps” that are noticeable in a few places on the full-size version of the composite image.

I cabled a Godox PROPAC PB960 to the Godox TT685C external flash unit that is used to backlight the white background. The power pack enables faster flash recycle times and increases the number of times the flash can be fired before its AA batteries run down. That was a big “hit!”

I didn’t realize the radio flash trigger was set for a power ratio of 1/4 +0.7 — that’s 2/3 of a stop slower than my preferred setting of 1/2 +0.3 that usually results in the pure white background (255, 255, 255) that is a goal of the “Meet Your Neighbours” (MYN) technique. That was a big “miss” I was able to correct in post-processing, although increasing the exposure enough to blow out the background might have degraded image quality a little.

One of many reasons the Fujifilm X-Series cameras are so popular is their retro look and feel, including lots of buttons and dials on the camera body. That’s good and bad: it’s good to be able to adjust many camera settings using either an external button or dial rather than navigating through menus in the camera’s firmware; it’s bad that it’s easy to change camera settings accidentally.

I must have rotated the back dial slightly because the shutter speed was set for 1/200 s rather than the camera sync speed of 1/250 s. Using a faster shutter speed can result in sharper images.

Related Resources

• Focus bracketing using Fujifilm X-T3
• RAW FILE CONVERTER EX 3.0
• Macromia illinoiensis exuvia (face-head)
• Macromia illinoiensis exuvia (face-head) redux

Copyright © 2020 Walter Sanford. All rights reserved.

Macromia illinoiensis exuvia (face-head) redux

A Swift River Cruiser dragonfly (Macromia illinoiensis) exuvia was collected, with permission from park staff, on 27 May 2017 along the Potomac River at Riverbend Park in Fairfax County, Virginia USA.

27 MAY 2017 | Riverbend Park | Swift River Cruiser (exuvia)

The preceding image shows the remnant ommatidia clearly.

From this viewpoint, it’s harder to see the prominent horn on the face that is a key field mark for larvae/exuviae in the Family Macromiidae (Cruisers). The base of the triangular horn is located above the labium (face mask), between the long, thin antennae; the apex of the triangle is pointed toward the viewer.

It’s easier to see the horn in the featured photo in my last blog post.

Tech Tips

The subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

20 photos of the specimen were taken using 2.5x magnification at an aperture of f/4; in-camera focus peaking was used to highlight select areas in each photo. RAW FILE CONVERTER EX 3.0 was used to convert Fujifilm RAF files to TIFF files. Adobe Photoshop CC 2017 was used to create a focus-stacked composite image that was edited using Apple Aperture.

Copyright © 2020 Walter Sanford. All rights reserved.

Macromia illinoiensis exuvia (face-head)

The following photograph of a Swift River Cruiser dragonfly (Macromia illinoiensis) exuvia shows a prominent horn on the face that is a key field mark for larvae/exuviae in the Family Macromiidae (Cruisers).

The specimen was collected, with permission from park staff, on 27 May 2017 along the Potomac River at Riverbend Park in Fairfax County, Virginia USA.

27 MAY 2017 | Riverbend Park | Swift River Cruiser (exuvia)

Tech Tips

The subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique. The specimen was posed on its dorsal side, so the camera was focused on the face-head-ventral view of the exuvia. The final image was cropped and rotated 180° during post-processing.

10 photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. RAW FILE CONVERTER EX 3.0 was used to convert Fujifilm RAF files to TIFF files. Adobe Photoshop CC 2017 was used to create a focus-stacked composite image that was edited using Apple Aperture.

Editor’s Note: This blog post is the last installment in what turned out to be a three-part series. The featured focus-stacked composite image is a little closer to what I had in mind when I set up the photo shoot.

1. MYN – Macromiidae larvae/exuviae are horny
2. RAW FILE CONVERTER EX 3.0

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Macromiidae larvae/exuviae are horny

A prominent horn on the face is a key field mark for larvae/exuviae in the Family Macromiidae (Cruisers), as shown in the following photograph of a Swift River Cruiser dragonfly (Macromia illinoiensis) exuvia.

The base of the triangular horn is located above the labium (face mask), between the long, thin antennae.

27 MAY 2017 | Riverbend Park | Swift River Cruiser (exuvia)

The specimen was collected, with permission from park staff, on 27 May 2017 along the Potomac River at Riverbend Park in Fairfax County, Virginia USA.

Tech Tips

The subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique. The specimen was posed on its dorsal side, so the camera was focused on the face-head-ventral view of the exuvia. The image was rotated 180° during post-processing.

My vintage 2009 Apple iMac desktop computer is too old to support drivers for importing RAF files from my Fujifilm X-T3 mirrorless digital camera into either Apple Aperture or Adobe Lightroom.

The work-around is to use a free application from Fujifilm that converts RAF files to TIFF files, which can be opened and edited with Aperture and Lightroom. Problem is I’m a big procrastinator and haven’t learned how to use the file converter application, so I simply edited one of the JPGs straight from the camera.

For the most part, the finished image looks fairly good although the eyes are blown out a little to a lot. JPG files have less dynamic range than RAF files so I was unable to recover the blown highlights.

Hmmm, it might be time to buy a new desktop computer!

Post Updates

I finally got around to figuring out how to use the free Fujifilm application to convert RAF files to TIFFs. See my follow-up blog post entitled “RAW FILE CONVERTER EX 3.0” for step-by-step instructions.

10 photos (converted from RAF to TIFF), including the one-off featured in this blog post, were used to create a focus-stacked composite image of the specimen that is a little closer to what I had in mind when I set up the photo shoot.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Hylogomphus adelphus exuvia (dorsal)

The following Anisoptera exuvia — collected by Freda van den Broek on 10 June 2019 along the St. Croix River in Polk County, Wisconsin USA — is definitely a member of Family Gomphidae (Clubtails), probably Mustached Clubtail (Hylogomphus adelphus).

Dorsal view

The abdomen is wider than the head. Vestigial mid-dorsal hooks are noticeable along several abdominal segments, especially segment nine (S9). A “median groove” is apparent along part of the abdomen. The lateral spine on S9 is spinulose-serrate along the outer edge.

10 JUN 2019 | Polk County, WI | H. adelphus exuvia (dorsal)

Related Resources

• MYN – Hylogomphus adelphus exuvia [face-head-dorsal]
• MYN – Hylogomphus adelphus exuvia (ventral)

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

15 photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create- and annotate the focus-stacked composite image shown above.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Hylogomphus adelphus exuvia (ventral)

The following Anisoptera exuvia — collected by Freda van den Broek on 10 June 2019 along the St. Croix River in Polk County, Wisconsin USA — is definitely a member of Family Gomphidae (Clubtails), probably Mustached Clubtail (Hylogomphus adelphus).

Ventral view

Notice the exuvia has a flat labium (prementum). There are lateral spines located on abdominal segments six through nine (S6-S9); the lateral spine on S9 is spinulose-serrate along the outer edge. Also notice the well-developed tibial burrowing hooks on its legs.

10 JUN 2019 | Polk County, WI | H. adelphus exuvia (ventral)

Male (vestigial genitalia)

Male odonates have two sets of sex organs: primary genitalia located on abdominal segment nine (S9); and secondary genitalia located on abdominal segments two-to-three (S2-3).

Both sets of vestigial genitalia are clearly visible on the ventral side of some (but not all) specimens, such as the H. adelphus exuvia shown above.

Related Resources

• MYN – Hylogomphus adelphus exuvia [face-head-dorsal]
• MYN – Hylogomphus adelphus exuvia (dorsal)

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

12 photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. A subset of six (6) photos and Adobe Photoshop CC 2017 were used to create- and annotate the focus-stacked composite image shown above.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Hylogomphus adelphus exuvia

An Anisoptera exuvia was collected by Freda van den Broek on 10 June 2019 along the St. Croix River in Polk County, Wisconsin USA.

This specimen is definitely a member of Family Gomphidae (Clubtails), probably Mustached Clubtail (Hylogomphus adelphus).

10 JUN 2019 | Polk County, WI | Anisoptera exuvia (face-head-dorsal)

Related Resources

• MYN – Hylogomphus adelphus exuvia (ventral)
• MYN – Hylogomphus adelphus exuvia (dorsal)

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique. This photo is a “one-off,” that is, not a composite image.

Eight (8) photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 will be used to create a focus-stacked composite image of the exuvia.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Too dark or too light?

The following Anisoptera exuvia — collected by Freda van den Broek on 10 June 2019 along the Red Cedar River in Barron County, Wisconsin USA — is definitely a member of Family Gomphidae (Clubtails), possibly Rusty Snaketail (Ophiogomphus rupinsulensis).

Dorsal-lateral view

Nine (9) photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown below.

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (dorsal–lateral)

When I previewed the RAW files used to create the preceding composite image, I thought most of the files look too dark.

So I did a do-over using 15 photos, resulting in a new version of the composite that is perfectly in focus but a little too light. Compare/contrast the two composite images and you should notice it’s easier to see the mid-dorsal hooks along the abdomen in the first image. The primary purpose for the images is to illustrate those field marks, therefore I think the first (darker) image is better.

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (dorsal–lateral)

Face-head-dorsal view

Five (5) photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown below.

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (face-head-dorsal)

My first impression of the version shown above is it’s too dark, and I second-guessed myself for not shooting more photos for the focus stack.

I created a new focus-stacked composite image using a little more light and 14 photos. When I examined the RAW files used to create the new composite image (shown below), I thought most of the files still look too dark. (But I was wrong.)

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (face-head-dorsal)

So I created a third version using much more light and 24 photos. Although it’s debatable which image looks best, the first- and second images show a few key field marks of the antennae better than the third image.

It turns out the antennae are one indicator this specimen is from the genus Ophiogomphus (Snaketails), so the first two (darker) images are the winners once again.

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (face-head-dorsal)

For what it’s worth, the second of three images is my favorite as indicated by the fact that the image is cropped so there’s room for annotations.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Clubtail exuvia (dorsal-lateral view)

The following Anisoptera exuvia — collected by Freda van den Broek on 10 June 2019 along the Red Cedar River in Barron County, Wisconsin USA — is definitely a member of Family Gomphidae (Clubtails), possibly Rusty Snaketail (Ophiogomphus rupinsulensis).

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (dorsal–lateral)

Notice the exuvia has little stubby mid-dorsal hooks along the length of its abdomen, and lateral spines on abdominal segments seven through nine (S7-S9).

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

15 photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown above.

Copyright © 2020 Walter Sanford. All rights reserved.

MYN – Clubtail exuvia (ventral view)

An Anisoptera exuvia was collected by Freda van den Broek on 10 June 2019 along the Red Cedar River in Barron County, Wisconsin USA.

This specimen is definitely a member of Family Gomphidae (Clubtails), possibly Rusty Snaketail (Ophiogomphus rupinsulensis).

10 JUN 2019 | Barron County, WI | Anisoptera exuvia (ventral)

Notice the exuvia has a flat labium (prementum). There are lateral spines located on abdominal segments seven through nine (S7-S9).

Tech Tips

This subject was photographed against a pure white background (255, 255, 255) using the “Meet Your Neighbours” (MYN) technique.

Seven (7) photos of the specimen were taken using an aperture of f/16; in-camera focus peaking was used to highlight select areas in each photo. Adobe Photoshop CC 2017 was used to create the focus-stacked composite image shown above.

Copyright © 2020 Walter Sanford. All rights reserved.